Compared to HIV cases, the symptoms observed in HIV-negative PJP patients are more severe and abrupt, and the clinical outcome is worse ( Guo et al., 2014), which indicates the significance of understanding the cellular and molecular basis of disease progression and developing better treatment strategies.ī cells have been demonstrated to play a vital role in host defense against Pneumocystis infection by antigen presentation and antibody production ( Kolls, 2017 Otieno-Odhiambo et al., 2019). The incidence of PJP has been increasing in patients without human immunodeficiency virus (HIV) infection due to the widespread use of immunosuppressive medications ( Bienvenu et al., 2016). Pneumocystis jirovecii pneumonia (PJP) is a severe opportunistic infectious disease found in immunocompromised patients ( Schmidt et al., 2018). Overall, these results present a detailed atlas of B cell transcriptional changes and BCR repertoire features in the context of Pneumocystis infection, which provides valuable information for finding diagnostic biomarkers and developing potential immunotherapeutic targets. Biased usage of V(D)J genes was observed, and the usage frequency of IGHV9-3 rose. Plasma cells exhibited higher levels of somatic hypermutation than naïve B cells. The proportion of clonal expanded cells progressively increased, while BCR diversity decreased. Moreover, Pneumocystis infection was associated with an increasing naïve B subset characterized by elevated expression of the transcription factor ATF3. We identified continuously increased plasma cells and an elevated ratio of (IgA + IgG) to (IgD + IgM) after infection. Here, we integrated single-cell RNA sequencing and single-cell BCR sequencing of immune cells from mouse lungs in an uninfected state and 1–4 weeks post-infection in order to illustrate the dynamic nature of B cell responses during Pneumocystis infection. However, the features of the B cell receptor (BCR) repertoire in disease progression remain unclear. B cells play vital roles in host defense against Pneumocystis infection.
0 kommentar(er)
